Extracellular vesicles: the new era of the intercellular communication

ROOM 5
WS.II EXTRACELLULAR VESICLES: The new era of the intercellular communication September 21
*Co-organized with:*

WORKSHOP COMMITTEE: Luciana DINI, Sapienza University of Rome Stefano TACCONI, Sapienza University of Rome & NanoShare Srl Annalisa RADEGHIERI, University of Brescia
Extracellular vesicles (EVs) have until recently been considered only cellular debris. Today they have been recognized as fundamental elements in intercellular communication. They are a heterogeneous population of membrane-bounded vesicles that play important roles not only in cellular communication but also in preventing or promoting certain diseases, including infectious diseases, neurological disorders, metabolic alterations, and cancer. With this session, we want to highlight two fundamental aspects in the study of EVs: the techniques and innovative approaches for EVs isolation and characterization and give some examples of their role in the onset of diseases. Considering the extreme heterogeneity of the EVs and the difficulty of establishing standard analytical procedures, in the first part of this session we will examine problems related to isolation, classification, and their characterization (morphological and biochemical). Furthermore, an example of isolation and use of EVs from milk will be provided as innovative possible carriers for the delivery of drugs or bioactive compounds. The second part of the workshop will focus on the role of EVs in the development and progression of diseases, like cancer, metabolic diseases and in the physiological maintenance of the extracellular matrix. The workshop will end with a symposium dedicated to young researchers in the field of extracellular vesicles. For further information about abstract and poster template, please write to Prof. Luciana Dini, This email address is being protected from spambots. You need JavaScript enabled to view it. and Dr. Stefano Tacconi, This email address is being protected from spambots. You need JavaScript enabled to view it.

September 21
09:00 - 10:30 Isolation and characterization of EVs WS.II.1 - TT.I.B
Chairs: Luciana DINI, Sapienza University of Rome & Stefano TACCONI, Sapienza University of Rome & NanoShare Srl
WS.II.1.1 TT.I.B.1	Rienk NIEUWLAND - CV Amsterdam UMC - University Medical Center, The Netherland A roadmap to improve the reproducibility of extracellular vesicle research
WS.II.1.2 TT.I.B.2	Simone DINARELLI - CV CNR-ISM High resolution atomic force microscopy as a tool for topographical mapping of surface budding
WS.II.1.3 TT.I.B.3	Piero DEL BOCCIO - CV The ‘Gabriele d’Annunzio’ University, Chieti-Pescara Proteomics characterization of FACS-sorted Extracellular Vesicles sub-types as liquid biopsy: new challenges in biomarkers discovery
WS.II.1.4 TT.I.B.4	Stefano TACCONI - CV Sapienza University of Rome & NanoShare Srl Bovine milk-derived Extracellular Vesicles as new drug delivery system for bioactive compounds

11:30 - 13:00 EVs in diseases WS.II.2 - TT.II.B
Chairs: Anna Maria GIUDETTI, University of Salento & Stefania MESCHINI, ISS
WS.II.2.1 TT.II.B.1	Maria FIANI - CV ISS Metabolically labeled exosomes for biogenesis and functional studies
WS.II.2.2 TT.II.B.2	Anna Maria GIUDETTI - CV University of Salento Insulin-resistant M2-CD163⁺ macrophages release extracellular vesicles affecting lipid metabolism in muscle cells
WS.II.2.3 TT.II.B.3	Flora GUERRA - CV University of Salento Extracellular vesicle secretion promotes cisplatin chemoresistance in a context of late endocytic impairment
WS.II.2.4 TT.II.B.4	Alberto DIASPRO - CV University of Genoa The era of the intelligent optical microscope towards extracellular vesicles visualization

14:00 - 15:30 PhD- derived EVs: implementing communication WS.II.3 - TT.III.B
BROCHURE
Chairs: Annalisa RADEGHIERI, University of Brescia & Massimo BOTTINI, University of Rome Tor Vergata
WS.II.3.1 TT.III.B.1	Massimo BOTTINI - CV University of Rome Tor Vergata Matrix vesicles: biochemical, biophysical, and biological properties
WS.II.3.2 TT.III.B.2	Emanuela FRUSTACI University of Rome Tor Vergata Biophysical and biochemical characterization of matrix vesicles
WS.II.3.3 TT.III.B.3	Lucas NOGUEIRA University of Rome Tor Vergata Matrix vesicle-embedded biopolymeric scaffolds: a model for in vivo bone mineralization studies
TT.III.B.4 WS.II.3.4	Rossella ZENATELLI University of Brescia EV-protein corona and EV surface engineering, a first study
TT.III.B.5 WS.II.3.5	Diana VARDANYAN University of Salento A step toward precision medicine using extracellular vesicles derived from different temozolomide-treated glioblastoma cells